CM-100020
HPLC Column EC 125/4.6 NUCLEODUR 100-5 NH2
This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions.
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Specifications
Pore Size: 100 Å
Style: Nucleodur
Particle Size: 5 µm
Mode: Reversed Phase (RP), Normal Phase (NP)
Type: (NH2) Amino
Carbon Load: 2.5
Length: 125 mm
Brand: Macherey-Nagel
Endcapped: No
Inner Diameter: 4.6 mm
Mfg. No.: 760720.46
Product Details
- Method HPLC, analytical
- Brand NUCLEODUR
- Base material NUCLEODUR - high purity silica
- Phase NUCLEODUR 100-5 NH2
- USP listing L8
- Surface chemistry Aminopropyl (NH2) modification
- Mode Multi-mode (NP and RP), Normal phase (NP)
- Type EC HPLC column (analytical)
- Hardware Stainless steel
- Column length 125 mm
- Column inner diameter 4.6 mm
- Particle type Fully porous particles (FPP)
- Particle shape Spherical
- Particle size 5 µm
- Eluent in HPLC column n-heptane
- Endcapped No
- Carbon content 2.5 %
- pH stability 2.0–8.0
- Pore size 110 Å
- Specific surface according to BET 340 m²/g
- Temperature stability (max) 60 °C
- Pressure stability (max) 600 bar / 8700 psi
- Column volume 2.06 mL
- Storage temperature 15–25 °C / 59–77 °F
- Recommended application(s) HPLC, Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases)

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