CM-100009
HPLC Column EC 125/4 NUCLEODUR 300-5 C4 125 mm, ID: 4 mm
Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR silica gel and is often used for the separation of biomolecules. Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.
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Specifications
Style: Nucleodur
Mode: Reversed Phase (RP)
Type: C4 (RP4, Butyl)
Length: 125 mm
Brand: Macherey-Nagel
Inner Diameter: 4 mm
Mfg. No.: 760194.40
Product Details
- Method HPLC, analytical
- Brand NUCLEODUR
- Base material NUCLEODUR - high purity silica
- Phase NUCLEODUR 300-5 C4 ec
- USP listing L26
- Surface chemistry Medium density monomeric butyl (C4) wide-pore modification
- Mode Reversed phase (RP)
- Type EC HPLC column (analytical)
- Hardware Stainless steel
- Column length 125 mm
- Column inner diameter 4 mm
- Particle type Fully porous particles (FPP)
- Particle shape Spherical
- Particle size 5 µm
- Eluent in HPLC column Acetonitrile – water
- Endcapped Yes
- Carbon content 2.5 %
- pH stability 1.0–9.0
- Pore size 300 Å
- Specific surface according to BET 100 m²/g
- Temperature stability (max) 60 °C
- Pressure stability (max) 450 bar / 6525 psi
- Column volume 1.56 mL
- Storage temperature 15–25 °C / 59–77 °F
- Recommended application(s) Biological macromolecules, HPLC, Peptides, Proteins


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